2. Apoptosis
  3. TNF Receptor
  4. N-Formyl-Met-Leu-Phe

N-Formyl-Met-Leu-Phe  (Synonyms: fMLP; N-Formyl-MLF)

目录号: HY-P0224 纯度: 99.90%
COA 产品使用指南 技术支持

N-Formyl-Met-Leu-Phe (fMLP; N-Formyl-MLF) 是一种趋化肽和N-甲酰基肽受体 (FPR) 的特异性配体。报道显示N-Formyl-Met-Leu-Ph 可抑制 TNF-alpha 的分泌。

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Custom Peptide Synthesis

N-Formyl-Met-Leu-Phe

N-Formyl-Met-Leu-Phe Chemical Structure

CAS No. : 59880-97-6

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规格 价格 是否有货 数量
5 mg ￥500
In-stock
10 mg ￥800
In-stock
25 mg ￥1320
In-stock
50 mg ￥1900
In-stock
100 mg ￥2660
In-stock
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500 mg   询价  

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Customer Review

N-Formyl-Met-Leu-Phe 相关抗体

Top Publications Citing Use of Products

查看 TNF Receptor 亚型特异性产品:

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TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

N-Formyl-Met-Leu-Phe (fMLP; N-Formyl-MLF) is a chemotactic peptide and a specific ligand of N-formyl peptide receptor (FPR). N-Formyl-Met-Leu-Ph is reported to inhibit TNF-alpha secretion.

IC50 & Target

TNF-alpha[1]
细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HL-60 EC50
0.01 μM
Compound: fMLF
Agonist activity at FPR1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
Agonist activity at FPR1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
[PMID: 19639995]
HL-60 EC50
1.9 μM
Compound: fMLF
Agonist activity at FPRL2 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
Agonist activity at FPRL2 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
[PMID: 19639995]
HL-60 EC50
20.4 μM
Compound: fMLF
Agonist activity at FPRL1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
Agonist activity at FPRL1 expressed in human HL60 cells assessed as induction of intracellular calcium flux by FLIPR3 calcium assay
[PMID: 19639995]
体外研究
(In Vitro)

N-Formyl-Met-Leu-Phe 与其特定细胞表面受体 N-甲酰基肽受体 (FPR) 的结合会触发不同的生化事件级联，最终导致细胞活化。FPR 是属于 G 蛋白偶联受体家族的趋化受体。N-Formyl-Met-Leu-Phe 在成骨细胞分化条件下促进成骨细胞定型并抑制脂肪形成定型。N-Formyl-Met-Leu-Phe 刺激成骨与成骨标记物和矿化的表达增加有关。N-Formyl-Met-Leu-Phe 抑制过氧化物酶体增殖物激活受体-γ1 的表达。N-Formyl-Met-Leu-Phe 刺激的成骨分化是通过 FPR1-磷脂酶 C/磷脂酶 D-Ca2+-钙调蛋白依赖性激酶 II-ERK-CREB 信号通路介导的[1]。
N-Formyl-Met-Leu-Phe 是一种细菌衍生肽，可在人外周血单核细胞中诱导促炎细胞因子基因表达。细菌产物 LPS 和 N-Formyl-Met-Leu-Phe 通过多种信号通路协同诱导炎症反应。TLR4、IKKβ-IκBα 和 NF-κB 信号通路通过 p65 核转位依赖机制参与 TNF-α 的协同诱导[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

N-Formyl-Met-Leu-Phe 相关抗体:
体内研究
(In Vivo)

N-Formyl-Met-Leu-Phe 促进斑马鱼和兔子的骨形成。在超过 80% 的 N-甲酰基-Met-Leu-Phe 处理的斑马鱼中，5 dpf 的骨骼发育明显。用 N-Formyl-Met-Leu-Phe 处理导致 Runx2 的表达增加。在 N-Formyl-Met-Leu-Phe 处理的颅骨中，骨髓腔广泛形成，覆盖骨的结缔组织致密，如骨膜[1]。N-Formyl-Met-Leu-Phe 介导钙卫蛋白从 PMN 体外 释放。它以剂量依赖性方式诱导钙卫蛋白从 PMN 中释放。在 N-Formyl-Met-Leu-Phe 浓度为 0.1-10.0 nM 时，至少保留 10% 的总 PMN 钙卫蛋白[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

437.55

Formula

C21H31N3O5S
CAS 号
性状

固体

颜色

White to off-white

Sequence

Formyl-Met-Leu-Phe
Sequence Shortening

Formyl-MLF
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 133.33 mg/mL (304.72 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2855 mL 11.4273 mL 22.8545 mL
5 mM 0.4571 mL 2.2855 mL 4.5709 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.90%

COA (287 KB) RP-HPLC (249 KB) MS (213 KB) 元素分析报告 (212 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[2]

Cells are cotransfected with either a dominant negative form of IκBα or a dominant negative form of IKKβ together with the NF-κB-dependent luciferase reporter plasmid. The plasmid pCMVβ is used as a control for transfection efficiency and this is monitored via the expression of β-galactosidase. Cells are transiently transfected with plasmids using DEAE-dextran. The transfected cells are cultivated for 48 h before a 6-h incubation in medium ±N-Formyl-Met-Leu-Phe, LPS, or N-Formyl-Met-Leu-Phe/LPS. Luciferase activity is determined by using the luciferase assay kit and a Monolight 3010 luminometer[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice: N-Formyl-Met-Leu-Phe is prepared in sterile PBS. Under the anesthesia, mice are intranasally treated with LPS (0.3 mg/kg) or N-Formyl-Met-Leu-Phe (0.5 mg/kg) or N-Formyl-Met-Leu-Phe and LPS in 50 μL of sterile PBS (control), BAL is performed by cannulating the trachea with sterilized PBS, and cells from BAL fluid are stained with Wright-Giemsa stain after cytocentrifuge. For TNF-α protein release, BAL fluid is collected and secreted TNF-α is measured by ELISA as described above[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

N-Formyl-Met-Leu-Phe 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2855 mL 11.4273 mL 22.8545 mL 57.1363 mL
5 mM 0.4571 mL 2.2855 mL 4.5709 mL 11.4273 mL
10 mM 0.2285 mL 1.1427 mL 2.2855 mL 5.7136 mL
15 mM 0.1524 mL 0.7618 mL 1.5236 mL 3.8091 mL
20 mM 0.1143 mL 0.5714 mL 1.1427 mL 2.8568 mL
25 mM 0.0914 mL 0.4571 mL 0.9142 mL 2.2855 mL
30 mM 0.0762 mL 0.3809 mL 0.7618 mL 1.9045 mL
40 mM 0.0571 mL 0.2857 mL 0.5714 mL 1.4284 mL
50 mM 0.0457 mL 0.2285 mL 0.4571 mL 1.1427 mL
60 mM 0.0381 mL 0.1905 mL 0.3809 mL 0.9523 mL
80 mM 0.0286 mL 0.1428 mL 0.2857 mL 0.7142 mL
100 mM 0.0229 mL 0.1143 mL 0.2285 mL 0.5714 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Formyl-Met-Leu-Phe59880-97-6fMLP N-Formyl-MLFTNF ReceptorTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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